Complete Genome Sequence of Bradyrhizobium japonicum ACCC 15027 - SRA

SRX26051161: Complete Genome Sequence of Bradyrhizobium japonicum ACCC 15027
1 PACBIO_SMRT (Sequel II) run: 40,952 spots, 214.6M bases, 137.4Mb downloads

Design: "5-10g genomic DNA was sheared into 10-15 Kb fragments using a g-TUBE device. Then library was constructed using the SMRTbell Express Template Preparation Kit 2.0. Briefly, the sheared fragments were through single-strand overhangs removing, DNA damage repair, end-repair, A-tailing, barcoded overhang adapters ligation. The library was quantified using Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, CA), and the size of the library was checked using an Agilent 2100 Bioanalyzer System. Subsequent steps were followed as per the manufactures instruction to prepare SMRTbell library. The library was sequenced using the Sequel II sequencing platform."

Submitted by: Biotechnology Research Institute

Study: Complete Genome Sequence of Bradyrhizobium japonicum ACCC 15027

PRJNA1159492 • SRP531978 • All experiments • All runs

show Abstracthide Abstract

Bradyrhizobium japonicum ACCC 15027 has a highly efficient capacity of nitrogen fixation and competitive nodulation, making it a valuable inoculant in agriculture for increasing crop yields. We report the complete genome sequence of ACCC15027.

Sample:

SAMN43576846 • SRS22621590 • All experiments • All runs

Organism: Bradyrhizobium japonicum

Library:

Name: Bradyrhizobium japonicum ACCC 15027

Instrument: Sequel II

Strategy: RNA-Seq

Source: GENOMIC SINGLE CELL

Selection: cDNA

Layout: SINGLE

Runs: 1 run, 40,952 spots, 214.6M bases, 137.4Mb

Run	# of Spots	# of Bases	Size	Published
SRR30629247	40,952	214.6M	137.4Mb	2024-09-11

ID:: 35160579

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